I used to think that Jesse Duke was the inventor of ethanol. After all, in the 1979 Georgia episode "High Octane" Jesse ran straight ethanol (aka moonshine) in Black Tillie.
But today I saw an episode of Beverly Hillbillies where Grannie started an ambulance service and Jed asked her why Duke, the hound dog, had a container of XXX tied onto his collar. (love the name Duke). Of course it was for medicinal purposes but Grannie also said she could put it in the ambulance if it ran out of gas. That was filmed in 1968 so I thought to myself: I guess Grannie invented ethanol.
Upon further thought I realized that 1979 wasn't the first time Jesse put moonshine in the tank. He told a story about doing it back in the old days, which would have been way before 1968. In fact, it would have been in the era of prohibition. So, Jesse still is the inventor of ethanol.
Moonshine was (and is) way more expensive than gasoline so I would suspect that the first person who actually tested it tried it in a small engine first. But who knows, maybe it was a desperate moonshiner who needed to get away in their "runner".